| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Clostridium paraputrificum | GCGGAAACTGGAGAAGAAGG | TCTTGCCCTTTTGCTGTTAGC | 58.56 | 59.39 | 164 | 176 |
100.00%
|
100.00%
|
| Primer 2 | Clostridium paraputrificum | GTGGCACCCCTAATGATGC | TGCAAATCTTTCTGCCATTGC | 58.58 | 58.31 | 158 | 168 |
100.00%
|
100.00%
|
| Primer 3 | Clostridium paraputrificum | CGTGGCACCCCTAATGATGC | TGCAAATCTTTCTGCCATTGC | 61.45 | 58.31 | 159 | 168 |
100.00%
|
100.00%
|
| Primer 4 | Clostridium paraputrificum | TGCAACAGTTTCAAGGGTGC | TAGCAACGTCTTCCGCTCC | 59.54 | 59.79 | 212 | 157 |
100.00%
|
100.00%
|
| Primer 5 | Clostridium paraputrificum | GCTTTGGTGGTGGCTATGC | GGCCTCATTCCCAAAAGTGC | 59.49 | 59.75 | 298 | 157 |
100.00%
|
100.00%
|
| Primer 6 | Clostridium paraputrificum | CCATCTTTTGGGGTTGCTCC | CGTCTTAGGATCTAGGGCAGC | 59.10 | 59.73 | 285 | 154 |
100.00%
|
100.00%
|
| Primer 7 | Clostridium paraputrificum | TGGGTGCTAAGAAGGCTCC | ACTTGTTAAGGCCTGCTTGG | 59.01 | 58.38 | 216 | 149 |
100.00%
|
100.00%
|
| Primer 8 | Clostridium paraputrificum | GCTGCGGATCAAGCAAAGAG | TGCAGCTTCATCCTCTACAGG | 59.90 | 59.52 | 267 | 148 |
100.00%
|
100.00%
|
| Primer 9 | Clostridium paraputrificum | GGGGAGTAAGGCTTACCATGG | TTTCTCAGCGTCCCCACAC | 59.86 | 59.93 | 265 | 147 |
100.00%
|
100.00%
|
| Primer 10 | Clostridium paraputrificum | AGGAGAAAACGGGAGCTGC | TGCTGTTGTCCACCTGATAGG | 60.00 | 59.72 | 285 | 146 |
100.00%
|
100.00%
|